The Illumina NexteraXT transposon protocol is a cost effective way to generate paired end libraries. However the resulting insert size is highly sensitive to the concentration of DNA used. and the variation of insert sizes is often large. One consequence of this is some fragments may have an insert shorter than the length of a single read. https://www.bekindtopets.com/best-price-Fuel-Tank-for-the-Coleman-BT200X-CT200U-Trail-CT200U-EX-Mini-Bikes-Blemished-p17069-hot-choice/
Assessment of Insert Sizes and Adapter Content in Fastq Data from NexteraXT Libraries
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